Application of the rapid lysine decarboxylase test for early isolation and detection of salmonellae in sewage and other wastewaters.
نویسنده
چکیده
A method for early isolation and detection of salmonellae in sewage and other wastewaters by using the rapid lysine decarboxylase test as a single biochemical reaction for screening the suspected Salmonella colonies is described. By this method, Salmonella isolation and identification can be completed within 2 to 3 days in contrast to the 5 to 7 days required for the conventional method.
منابع مشابه
Development of selective lysine decarboxylase broth using spectrophotometric assay for Salmonella screening
This study was to demonstrate the application of spectrophotometry for rapid and presumptive differentiation of Salmonella from other Enterobacteriaceae based on lysine decarboxylase (LDC) activity. The pH indicator system and cocktails of Salmonella selective inhibitors were optimized to derive a new selective broth (patent pending no. 1301006577) for Salmonella screening. Phenol red (PR) was ...
متن کاملAssessment of a rapid immunochromatographic assay for the detection of avian influenza viruses
Rapid spreading of the low pathogenic avian influenza virus (AIV) caused by the H9N2 subtype and the highly pathogenic AIV caused by H5N1 have caused serious economic losses in the poultry industries of Asia. Therefore, the early detection of AIVs is crucial for the control of the disease. In the present study, the applicability of a rapid immunochromatographic (RIC) assay, which specifically d...
متن کاملDistribution of Enterococcal Species and Detection of Vancomycin Resistance Genes by Multiplex PCR in Tehran Sewage
Enterococci are important because of their role as the leading cause of nosocomial infections which have a significant role in the dissemination and persistence of antimicrobial resistance genes. Methods: In this study, we determined the distribution of enterococcal species in the sewage treatment plants in Iran. Furthermore, we improved a rapid and specific PCR method using primers (sodA and ...
متن کاملA Recommended Method for Detecting Salmonellae in Composted Biosolids
It has been found in Australia and in the United States that composting does not always result in the complete removal of salmonellae from biosolids. It is therefore likely that monitoring of composted biosolids for salmonellae will be required in Australia to ensure the safety ofbiosolids products. At present rapid methods of detection such as PCR and ELISA are not sufficiently developed to mo...
متن کاملIsolation and identification of Salmonellae from chicken carcasses in processing plants in Yazd province, central Iran
This study was conducted to determine the prevalence of Salmonellae contamination of chicken carcasses in slaughterhouses in central Iran (Yazd province). 435 samples were obtained from liver, breast— before and after chilling—and bacteriological and serological examinations were done. The results showed that the rate of contamination of liver, breast meat—before and after chiller—were 8.1, 18....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 34 4 شماره
صفحات -
تاریخ انتشار 1977